Genotyping of Clostridium perfringens isolates collected from food poisoning outbreaks and healthy individuals in Japan based on the cpe locus.

نویسندگان

  • Daisuke Tanaka
  • Keiko Kimata
  • Miwako Shimizu
  • Junko Isobe
  • Masanori Watahiki
  • Tadahiro Karasawa
  • Takayoshi Yamagishi
  • Sanae Kuramoto
  • Toshihiko Serikawa
  • Fubito Ishiguro
  • Makiko Yamada
  • Kazukiyo Yamaoka
  • Mitsuo Tokoro
  • Toshio Fukao
  • Masakado Matsumoto
  • Reiji Hiramatsu
  • Chie Monma
  • Yoshiyuki Nagai
چکیده

Daisuke Tanaka*, Keiko Kimata, Miwako Shimizu, Junko Isobe, Masanori Watahiki, Tadahiro Karasawa, Takayoshi Yamagishi, Sanae Kuramoto, Toshihiko Serikawa, Fubito Ishiguro, Makiko Yamada, Kazukiyo Yamaoka, Mitsuo Tokoro, Toshio Fukao, Masakado Matsumoto, Reiji Hiramatsu, Chie Monma and Yoshiyuki Nagai Toyama Institute of Health, Toyama 939-0363; Department of Clinical Laboratory Science, Kanazawa University Graduate School of Medical Science, Ishikawa 920-0942; Ishikawa Prefectural Institute of Public Health and Environmental Science, Ishikawa 920-1154; Fukui Prefectural Institute of Public Health and Environmental Science, Fukui 910-8551; Gifu Prefectural Institute of Health and Environmental Sciences, Gifu 504-0838; Gifu Municipal Institute of Public Health, Gifu 500-8881; Aichi Prefectural Institute of Public Health, Aichi 462-8576; and Tokyo Metropolitan Research Laboratory of Public Health, Tokyo 169-0073, Japan

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Enterotoxigenic Clostridium perfringens: Detection and Identification

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Multiplex PCR genotyping assay that distinguishes between isolates of Clostridium perfringens type A carrying a chromosomal enterotoxin gene (cpe) locus, a plasmid cpe locus with an IS1470-like sequence, or a plasmid cpe locus with an IS1151 sequence.

Clostridium perfringens type A isolates carrying the enterotoxin (cpe) gene are important causes of both food poisoning and non-food-borne diarrheas in humans. In North America and Europe, food poisoning isolates were previously shown to carry a chromosomal cpe gene, while non-food-borne gastrointestinal (GI) disease isolates from those two geographic locations were found to have a plasmid cpe ...

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Prevalence and characterization of enterotoxin gene-carrying Clostridium perfringens isolates from retail meat products in Japan.

Clostridium perfringens is an important anaerobic pathogen causing food-borne gastrointestinal (GI) diseases in humans and animals. It is thought that C. perfringens food poisoning isolates typically carry the enterotoxin gene (cpe) on their chromosome, while isolates from other GI diseases, such as antibiotic-associated diarrhea, carry cpe on a transferable plasmid. However, food-borne GI dise...

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Identification of Novel Clostridium perfringens Type E Strains That Carry an Iota Toxin Plasmid with a Functional Enterotoxin Gene

Clostridium perfringens enterotoxin (CPE) is a major virulence factor for human gastrointestinal diseases, such as food poisoning and antibiotic associated diarrhea. The CPE-encoding gene (cpe) can be chromosomal or plasmid-borne. Recent development of conventional PCR cpe-genotyping assays makes it possible to identify cpe location (chromosomal or plasmid) in type A isolates. Initial studies f...

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عنوان ژورنال:
  • Japanese journal of infectious diseases

دوره 60 1  شماره 

صفحات  -

تاریخ انتشار 2007